The antitumor activities of the cell bound polysaccharide(CBP), water soluble polysaccharide(WSP) and sulfated polysaccharide(SP) of Zoogloea sp. were observed. The results obtained were as follows : 1) Th CBP, WSP, and SP showed cytotoxic effect
on the
Meth A cells in vitro, however, the effect of CBP and WSP was more ten-fold greater than that of SP. 2) When CBP, WSP, and SP ws inoculated into the peritoneal cavity of the Meth A cells transplanted mce, the average survival days tended to
prolonged
slightly as compared with the control. 3)When Meth A cells were trasplanted subcutaneously into the back of mice, and then CBP, WSP, and SP was inoculated into the peritoneal cavity of mice, the tumor growth inhibition ratio was 46.9% for WSP,
40.4% for
CBP, and 16.2% for Sp. 4) The phagocytic activity of peritoneal macrophages elicited with CBP, WSP, and SP was significantly increased than that of control. 5) The production of nitric oxide in the peritoneal macrophages stimulated with CBP, WSP,
SP and
LPS aloneo was not increased than that of control. The production of nitric oxide in the peritoneal macrophages stimulated with IFN-r and CBP, IFN-r and WSP, and IFN-r and SP was significantly increased than that of control, but in the case of
stimulated with IFN-r and WSP was increased 50% for CBP and SP.
These results suggest that the CBP, WSP and SP of Zoogloea sp. showed direct cytotoxic effect and tumor growth inhibition on Meth A cells in vitro and in vivo, and induced nitric oxide production of activated macrophages.
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